Recent Advances in Development of Affinity Sensors based on Conducting Polymers

Description:
Conducting polymers are synthesized by various methods, namely by electrochemical [1], chemical [2] and biochemical [3-5]. Electrochemically generated conducting polymer based layers are applied in the design of various types of electrochemical biosensors and biofuel cells [6]. Conducting polymers are often applied in enzymatic (e.g. in glucose biosensors). In some we have demonstrated, that some enzymes [3] and living cells [5] can be applied in the synthesis of some conducting polymers, namely: polypyrrole [3], polyaniline [4], polytiophene and some other conducting polymer based layers and nanoparticles. We have demonstrated that during this kind of synthesis of nanoparticles and/or nanostructured layers the enzymes become entrapped within conducting polymer layer. In some researches we showed that redox processes, which are part of metabolism of living cells, can be applied for the synthesis of conducting polymer – polypyrrole (Ppy), and Ppy nanoparticles can be entrapped within cells [5]. The applicability of electrochemically generated polymers in molecularly imprinted polymers based sensors [6] will be discussed.
References
1. A. Ramanavicius, et al. Electrochemical Formation of Polypyrrole-based Layer for Immunosensor Design. Sens Actuat B 2014, 197, 237
2. K. Leonavicius, et al. Polymerization Model for Hydrogen Peroxide Initiated Synthesis of Polypyrrole Nanoparticles. Langmuir 2011, 17, 10970
3. A. Ramanavicius, et al. Self-encapsulation of oxidases as a basic approach to tune upper detection limit of amperometric bosensors. Analyst 2008, 133, 1083
4. A. Ramanavicius, et al. Synthesis of Polypyrrole Within the Cell Wall of Yeast by Redox-Cycling of [Fe(CN)6]3-/[Fe(CN)6]4-. Enzyme Microb Technol 2016, 83, 40
5. S. Ramanavicius, et al. Charge transfer and biocompatibility aspects in conducting polymers... Nanomaterials 2021, 11, 371.
6. S. Ramanavicius, et al. Advances in molecularly imprinted polymers based affinity sensors. Polymers 2021, 13, 974

Speaker: Arunas Ramanavicius - Vilnius University
Prof. Habil. Dr. Arunas Ramanavicius is a professor at Vilnius University, Vilnius, Lithuania. He is also leading the laboratory of NanoBioTechnology at Research Center of Physical Sciences and Technologies. Prof. Arunas Ramanavicius is a member of Lithuanian Academy of sciences. In 1998 he received PhD degree and in 2002 doctor habilitus degree from Vilnius University. He has research interests in various aspects of nanotechnology, bionanotechnology, nanomaterials, biosensorics, bioelectronics, biofuel cells and MEMS based analytical devices. He was a national coordinator of several nanotechnology related COST actions. Editorial board member of Materials; Sensors; Chemija and several other journals. Prof. A. Ramanavicius is serving as expert-evaluator in Horizon 2020 program coordinated by European Commission and he is technical advisor of many foundations located in European and non-European countries.