Facilitating High Throughput Food Determinations with SPME Technologies

Description:
The current sample preparation techniques used in the multiclass multi-residue analysis of pesticides in fruits and vegetables as well as veterinary drugs in animal tissue involve time-consuming procedures that are not always effective at minimizing matrix interferences. These methods often involve the use of large amounts of organic solvents which lead to hazardous waste. Moreover, they lack automation and high-throughput capabilities. Screening of contaminants present in food and the origin of food is an important task. New developments in high throughput determinations facilitated by SPME have been demonstrated not only with GC/MS and LC/MS but with direct couplings to MS. These are critical advances that will impact the effectiveness of public protection. In this presentation, we will describe two alternative approaches for multiclass multi-residue analysis based on solid phase microextraction. The first approach is based on conventional liquid chromatography-tandem mass spectrometry methods (LC-MS/MS). The second approach is based on the emerging direct analysis MS techniques. In both approaches, the main goal is aimed at minimizing matrix effects and organic solvent use, while maximizing sample throughput. The fully automated sample preparation workflow allows for a total extraction time of less than 1 min per sample when 96 extractions are simultaneously conducted, while the direct to MS workflow allows for a total analysis time of less than 1 min per sample with screening in both negative and positive ionization modes in the Coated Blade Spray (CBS) method. Strategies for the determination of hydrophobic pesticides will be discussed as well. All methods were able to achieve excellent accuracy and precision results.

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Speaker: Janusz Pawliszyn - University of Waterloo
The primary focus of Professor Pawliszyn's research program is the design of highly automated and integrated instrumentation for isolating analytes from complex matrices and the subsequent separation, identification, and determination of these species. He is a Fellow of the Royal Society of Canada and Chemical Institute of Canada, editor of Analytica Chimica Acta, Editor-in-Chief of Trends in Analytical Chemistry and a member of the Editorial Boards of Journal of Separation Science, Separations and Journal of Pharmaceutical Analysis. He initiated a conference, “ExTech”, focusing on new advances in sample preparation and disseminating new scientific developments in the area, which meets every year in a different part of the world.