Reduced Ion Suppression in an Automated Extraction of Vitamins B1 and B6 from Whole Blood in LCMS Analysis.

Description:
Phospholipids and proteins in blood and plasma samples are a main cause of ion suppression in LCMS analysis. In addition to ion suppression, the phospholipids and proteins in blood and plasma samples can cause premature column failures and increase frequency of instrument maintenance. Presented is the work demonstrating the effectiveness of the Biotage ISOLUTE PLD+™ phospholipid removal plate used in the extraction of Vitamins B1 and B6 from human whole blood. The PLD+ plate can be incorporated into an existing method with little or no modification, as the blood or plasma is precipitated directly on the plate and can be easily automated. The Biotage ISOLUTE PLD+™ demonstrated an over 90% reduction in phospholipids and proteins and an increase in signal to noise ratio of the endogenous levels of B1 and B6.