Analyzing protein post-translational diversity by data-independent acquisition mass spectrometry

Description:
The proteins, once synthesized, could undergo both protein post-translational modifications (PTMs) and protein turnover, providing the molecular basis shaping the biological diversity. The Liu Lab is working on measuring both protein PTMs and turnover rates by developing and applying consistent proteomic methods such as data-independent acquisition mass spectrometry (or DIA-MS). In the last five years, we and others have demonstrated that the MS2-based DIA-MS can impressively achieve higher reproducibility and accuracy than the traditional MS1-based data-dependent acquisition (DDA) in quantifying SILAC ratios, protein turnover, and phosphorylation regulation. We will discuss how protein post-translational diversity can be quantified and dissected in different cell systems, including cancer aneuploidy and longitudinal phosphorylation-mediated signaling transduction, to derive quantitative biological insights.

Speaker: Yansheng Liu - Yale University School of Medicine
Dr. Yansheng Liu is an Assistant Professor in the Department of Pharmacology at Yale University School of Medicine. He is also a group leader of quantitative proteomics at Yale Cancer Biology Institute. Dr. Liu received his Ph.D. in Biomedical Sciences from the Chinese Academy of Sciences in 2011. He then completed a 6.5-year post-doctoral training in the laboratory of Dr. Ruedi Aebersold at ETH Zurich, Switzerland. Dr. Liu joined the faculty at Yale in December 2017. Dr. Liu has particular research interests in protein post-translational diversity, cancer aneuploidy, and cell signaling transduction. The Liu Lab additionally aims to contribute to the development of multiplexed, high-throughput data-independent acquisition mass spectrometry (DIA-MS) technique. Dr. Liu has co-authored 62 publications with a total citation of more than 6200 times. Dr. Liu has received the 2021 ASMS research award and the 2021 HUPO ECR award.