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Making sense of microbial systems at the single cell level using simultaneous Raman and infrared spectroscopy.
Description:
Most analytical systems require large amounts of bacterial biomass in order to generate robust signals. This means that these signals are only indicative of the population of bacteria and not the individuals within a microbial community. Rod shaped bacteria typically have dimensions of 1x2 µm and a single bacterial cell weighs a mere 1 pg and so analysis using physicochemical methods at the single cell level is challenging. We have therefore been exploiting optical photothermal infrared (O-PTIR) spectroscopy, coupled with Raman spectroscopy, as this approach allows these complementary vibrational fingerprints to be taken from the same place from single bacterial cells. In addition, we have also been exploiting the non-linear Raman method of stimulated Raman spectroscopy for similar purposes.
This presentation will relate our developments in this area where we have (i) achieved bacterial identification of pathogens that cause sepsis at the single level (https://doi.org/10.1039/d2sc02493d); (ii) are able to probe metabolism of bacteria at the single cell level (https://dx.doi.org/10.1021/acs.analchem.0c03967; https://doi.org/10.3390/s22103928); and (iii) probe the effects of microplastics on biological systems.
Speaker: Roy Goodacre - University of Liverpool
Co-Authors
Making sense of microbial systems at the single cell level using simultaneous Raman and infrared spectroscopy.