Massively Multiplexed Imaging of Human Pathology
Description:
Spatial proteomics, starting with the earliest low parameter fluorescent cytometry and microscopy experiments, helped define the major cell subsets and architecture of human tissues as we understand them today. Now, a novel combination of elemental mass spectrometry with nanometer-scale imaging (multiplexed ionbeam imaging – MIBI, Nature Med. 2014, Cell 2018, Science Adv., 2019) offers routine, simultaneous quantification of > 40 proteomic features without fluorescent agents or interference from spectral overlap and autofluorescence using heavy metal isotopes as reporters. With this, we have reached new levels of understanding in tissue immune organization, combined with novel single-cell visualization and analysis methods. By identifying new cell populations, regulatory relationships, and structural rulesets we have identified numerous clinically predictive features underlying human disease.
Neurodegenerative disease represents one of the most technically challenging topics to address with next-generation spatial -omics in general. Facets of these diseases are largely human specific and characterized human central nervous system (CNS) tissues are difficult to work with in their archival format. Here, we highlight early applications of MIBI technology to resolve human neurodegenerative disease and cognitive resilience in retrospective studies of the human CNS. Our analyses reveal unappreciated layers of human cellular organization and structure in these systems that can be exploited to understand and predict disease pathobiology.
Speaker: Sean Bendall - Stanford University School of Medicine
Sean C. Bendall is an Associate Professor in the Department of Pathology at Stanford University, School of Medicine. His research specialty is the development and application of single-cell proteomic tools for the investigation of human systems. This includes pioneering single cell CyTOF mass cytometry and multiplexed ion beam imaging (MIBI). He is also a co-founder of IONpath which is commercializing MIBI technology for pathobiology and research services. Sean’s work in single cell biology has provided unparalleled granularity of
Co-Authors
Massively Multiplexed Imaging of Human Pathology
Category
2023 Call for Invited Abstracts
Description
Session Number: Waters-03
Session Type: Symposium
Session Date: Monday 3/20/2023
Session Time: 1:30 PM - 4:50 PM
Room Number: 126A
Track: Bioanalytics & Life Sciences
Category: Bioanalytical
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