Using secondary ion mass spectrometry and matrix assisted laser desorption / ionization mass spectrometry for spatially resolved and single cell chemical characterization of tissues and biofilms
Description:
Mass spectrometry imaging of biological samples has become an important tool for spatially resolved untargeted chemical measurements. Buckminsterfullerene (C60) secondary ion mass spectrometry (SIMS) excels at detection of intact low molecular weight ions with little sample preparation. The rich spatial and chemical information provided by C60-SIMS can be expanded by imaging the same surfaces with confocal Raman microscopy, or even with other MS approaches such as matrix assisted laser desorption / ionization (MALDI) mass spectrometry (MS). Several examples of these combinations are highlighted including the study of Pseudomonas aeruginosa biofilms and the rodent brain. In addition to mass spectrometry imaging, approaches for assaying the chemical content within individual cells are explored with both SIMS and MALDI MS. Using these approaches, we can measure lipids, fatty acids, and peptides, among others. For single cell measurements, the cells of interest are scattered across a microscope slide, the exact cell positions determined via optical microscopy, and the mass spectra are acquired only at the desired cell positions. Because the spaces between the cells are not measured, the approach is efficient and rapid. By obtaining information from thousands of individual cells, rare cells are found and unusual metabolites discovered. We are currently adapting the approach to work with individual cellular organelles such as dense core vesicles and mitochondria providing us with more detail on organelle heterogeneity. This has allowed us to characterize distinct populations of vesicles that contain varying contents.
Speaker: Jonathan Sweedler - University of Illinois
Jonathan Sweedler received his Ph.D. in Chemistry from the University of Arizona in 1988, spent several years at Stanford before moving to the University of Illinois at Urbana-Champaign in 1991 where he has been ever since. He is currently the James R. Eiszner Family Endowed Chair in Chemistry. His research interests focus on developing new approaches for assaying small volume samples, including metabolomics and peptidomics, based on mass spectrometry. He has used these tools to characterize small molecules and peptides in a range of animal models across the metazoan and in samples as small as individual cells and cellular domains.
Sweedler has published more than 400 manuscripts and presented 500 invited lectures. He has received numerous awards including the ACS Award in Analytical Chemistry and the Pittsburgh Analytical Chemistry Award. He is currently the Editor-in-Chief for Analytical Chemistry.
Co-Authors
Using secondary ion mass spectrometry and matrix assisted laser desorption / ionization mass spectrometry for spatially resolved and single cell chemical characterization of tissues and biofilms
Category
2023 Call for Invited Abstracts
Description
Session Number: Waters-05
Session Type: Award Abstract
Session Date: Monday 3/20/2023
Session Time: 1:30 PM - 4:50 PM
Room Number: 126A
Track: Bioanalytics & Life Sciences
Category: Bioanalytical, Mass Spectrometry, Metabolomics/Microbiome
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