High-Sensitivity, High-Specificity Detection with DNA Nanoswitches

Description:
Despite recent advances, there remains a need for a detection method that combines the simplicity and cost-effectiveness of ELISA with the sensitivity and speed of modern approaches in a format suitable for rapid, point-of-care applications. Building on recent developments in DNA structural nanotechnology, we have developed the nanoswitch-linked immunosorbent assay (NLISA), a detection platform based on easily constructed DNA nanodevices that change conformation upon binding to a target protein. NLISA is surface-free and includes a kinetic-proofreading step for purification, enabling both enhanced sensitivity and reduced cross-reactivity. We have demonstrated femtomolar-level detection of proteins in biological fluids, as well as multiplexed detection of both nucleic acid and protein biomarkers in one pot with a single unified workflow. We have also been developing a new approach for single-molecule protein identification, which we will discuss if time permits.

Speaker: Wesley Wong - Harvard Medical School