Cannabis Isolation and Identification Workflow using Flash and HPLC

Description:
Cannabis. A major topic of discussion over the past several years especially since the US Farm Bill of 2018 legalized cannabidiol (CBD) and myriad other non-psychoactive cannabinoids isolated from hemp. Since their legalization, it has been up to individual states to set their regulatory standards while nationally, a maximum THC limit of 0.3% in the final marketed product was set.
This meant that every hemp processor and handler from the biomass through end-product needed a means of testing for cannabinoid ID and its amount to ensure compliance with the THC level, cannabinoid potency, and determination other impurities (and their levels) such as pesticides, fungicides, aflatoxins, and endotoxins. Most have turned to using external analytical labs for these analyses but in order to provide a purified cannabinoid, the processor is required to isolate the cannabinoid or cannabinoids needed. For many hemp processors, purification of these compounds is best accomplished with reversed-phase flash chromatography using a simple and efficient step gradient.
In this presentation we will discuss some of the workflow process analytical labs may employ in order to isolate and analyze individual cannabinoids in order to determine cannabinoid ID to meet their customer’s need and even provide standards which, if commercially available, may be quite expensive.

Speaker: Michael Ebitson - Biotage
Currently Michael Ebitson is the Director of Applications at Biotage. Michael is based in Salem Nh and supports Sales and marketing's Pre and Post sales workflow solutions. Michael enjoys working closely with customers using his knowledge and experience to assist them in overcoming their laboratory challenges and workflow hurdles. Michael has been practicing analytical chemistry for over 23 years. With his environmental biology and chemistry education coupled with his extensive experience he has developed and supported new and existing chemistry solutions and products for customers in markets such as Pharmaceutical, Agriculture, Supplements and Environmental Testing. Prior to his current role Michael had worked for ten years in environmental testing. As a customer and a manager in analytical testing Michael truly understands laboratory needs and the workflow hurdles that are experienced daily with complex matrices.