Mastering UPLC and HPLC: Beginner to Expert in One Day!
Description:
HPLC is one of the most popular analytical tools in the world, yet very few people understand how it really works. During this 1-day course, you will not only understand it, but you will also master it! We'll start with a review of the fundamentals of HPLC/UPLC, and then I'll show you how to use those fundamentals to develop and optimize methods, cut your analysis time in half, and troubleshoot your instruments. My goal is to put you into the driver's seat, so you are in control of your HPLC and not the other way around!
I have been teaching HPLC to professionals for 30 years. What I have learned in that time is that most people who operate an HPLC, have little to no idea how it works. The pleasant surprise is that it is completely understandable.
IF you want to understand HPLC, you will by the end of this course!
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Instructor: Lee Polite - Axion Analytical Labs, Inc
Biography: For the last 25 years, Dr. Polite has taught chromatography to more than 12,000 satisfied scientists around the world, including employees from every major pharmaceutical, chemical and petroleum company in the US. Lee received his PhD in chromatography from Virginia Tech under the direction of Professor Harold McNair. Dr. Polite spent 9 years as a research scientist and group leader for a major petrochemical company, before starting Axion Analytical Labs and Axion Training Institute in Chicago, where he and his staff teach week-long hands-on HPLC and GC courses.
Course Outline:
Introduction to Liquid Chromatography
What is UPLC? HPLC? RPLC?
Resolution - The only important parameter in HPLC! Understanding the separation parameters and how to apply them: You are in control of the entire separation!
HPLC Instrumentation - Understanding the nuts and bolts from the solvent bottles to the detector waste line.
Method Development - You will learn how to develop an HPLC/UPLC method from scratch!
Method Optimization - Learn how to cut your analysis time in half...guaranteed!
Troubleshooting Instrument Problems from the Chromatograms
Learning Objectives:
After this course, you will know how to:
• develop a method from scratch, including choosing the correct solvent strength, column length, particle diameter, stationary phase, temperature and flow rate!
• Cut your analysis time in half, without affecting the resolution!
• Cut your analysis time by a factor of 10!
• Improve your sensitivity 10-1000 fold
• Improve your separation
• Be able to explain to somebody what HPLC is, and how it works.
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Mastering UPLC and HPLC: Beginner to Expert in One Day!
Lee Polite - Axion Analytical Labs, Inc
Mastering UPLC and HPLC: Beginner to Expert in One Day!
Description
Session Number: SC294
Session Type: Short Courses
Session Date: Sunday 3/19/2023
Session Time: 8:30 AM - 5:00 PM
Room Number: Short Course Office 108B
Track: Professional Skills Building
Category: HPLC, Liquid Chromatography, Liquid Chromatography/Mass Spectrometry
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