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Biography: Dr. Perry G. Wang has been a chemist at US FDA since 2008. Prior to joining the FDA, he worked in the pharmaceutical and medical-device industry for more than 10 years. He received his Ph.D. from Oregon State University. He specializes in LC-MS method development and validation for drugs, cosmetics, foods, and dietary supplements. He has been invited to teach this course at Pittcon since 2007. He has also taught this course for the American Chemical Society (ACS), Eastern Analytical Symposium (EAS), American Society for Mass Spectrometry, and HPLC. He teaches these courses in his own capacity as a scientist, but not as an employee of the FDA.

Course Outline:
1. HPLC principles
Retention time (tR)
Retention factor (k’)
Separation factor (α)
Column efficiency (N)
Resolution (R)
2. Separation modes and mechanism
Reversed-phase and normal-phase
How and when pH affects the separation
3. LC method development
Isocratic or gradient
How and when pH affects the separation
How to transfer HPLC methods to UHPLC/UPLC methods
4. MS principles
What an MS can tell you
How to make ions
Ionization modes: ESI, APCI and APPI
How to separate ions
How to build up a mass chromatogram
Mass resolution and mass accuracy
5. MS method build-up
What kind of ionization should be selected – ESI, APPI or APCI
How to find precursor and product ions
Single and triple quadrupole, TOF, ion trap and Q-exactive
Mechanisms of matrix effects in ESI, APCI and APPI
How to evaluate and quantify the matrix effect
How to eliminate or compensate for matrix effects
How to “balance” sensitivity and peak accuracy
Scheduled SRM to improve sensitivity
How to optimize an LC-MS/MS method

Learning Objectives:
After this course, the participants will be able to independently develop their own LC-MS/MS methods to use in their laboratories.

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